lymphoprep density gradient medium (STEMCELL Technologies Inc)

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STEMCELL Technologies Inc lymphoprep density gradient medium
Lymphoprep Density Gradient Medium, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lymphoprep+density+gradient+medium/lymphoprep/pmc12164180-197-11-17
Average 90 stars, based on 1 article reviews

lymphoprep density gradient medium - by Bioz Stars, 2026-09

90/100 stars

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Isolation:

Article Title: Activation of endogenous full-length utrophin by MyoAAV-UA as a therapeutic approach for Duchenne muscular dystrophy
Article Snippet: .. PBMCs from two male DMD patients were isolated from the peripheral blood using 15 ml SepMate tubes (STEMCELL, #15450) and Lymphoprep density gradient medium (STEMCELL, #07801). .. All cell culture-related experiments involving DMD patient’s PBMCs were conducted in compliance with the Chinese PLA General Hospital Ethics Committee guidelines (Approval number KY2121-001). iPSCs were generated from PBMCs using the Invitrogen Sendai virus kit (Invitrogen, #A16518).

Article Title: Adjuvant conditioning shapes the adaptive immune response promoting immunotolerance via NLRP3/interleukin-1
Article Snippet: .. PBMCs were isolated using Lymphoprep density gradient medium centrifugation (Stemcell Technologies). ..

Gradient Centrifugation:

Article Title: CFTR negatively reprograms Th2 cell responses and CFTR potentiation restrains allergic airway inflammation.
Article Snippet: .. For human cell CD4+ T cell studies, human PBMCs were prepared from whole blood of healthy donors by Ficoll density gradient centrifugation using Lymphoprep density gradient medium (StemCell Technologies) and SepMate-50 tubes (StemCell Technologies). .. For the purification of human naive CD4+ T cells, an EasySep human naive CD4+ T cell isolation kit II (StemCell Technologies) was used.

Article Title: CFTR negatively reprograms Th2 cell responses, and CFTR potentiation restrains allergic airway inflammation
Article Snippet: For humanized CFTR CD4 + T cell studies, cells from hCFTR ΔF508 and hCFTR mice were isolated and polarized in Th2 conditions as above, in the presence of the CFTR potentiator ivacaftor (1 μM; Selleck Chemicals), and the CFTR correctors elexacaftor (3 μM; Selleck Chemicals) and tezacaftor (3 μM; Selleck Chemicals, elexacaftor-tezacaftor-ivacaftor [ETI]) or DMSO control (%5 v/v). .. For human cell CD4 + T cell studies, human PBMCs were prepared from whole blood of healthy donors by Ficoll density gradient centrifugation (800 g ) using Lymphoprep density gradient medium (StemCell Technologies) and SepMate-50 tubes (StemCell Technologies). .. For the purification of human naive CD4 + T cells, an EasySep human naive CD4 + T cell isolation kit II (StemCell Technologies) was used.

Centrifugation:

Article Title: Adjuvant conditioning shapes the adaptive immune response promoting immunotolerance via NLRP3/interleukin-1
Article Snippet: 2ME (2-Mercaptoethanol) , Gibco , 21985023. .. Lymphoprep density gradient medium centrifugation , STEMCELL Technologies , 07801. .. EDTA , Sigma-Aldrich , E9884.

other:

Article Title: Dietary grape pomace ameliorates intestinal damage and oxidative stress by modulating MAPK-Nrf2/ARE pathways in coccidia-challenged broilers ( Gallus gallus )
Article Snippet: The diluted blood was then gently layered onto a 4 mL Lymphoprep TM density gradient medium (1.077g/mL, Stemcell Technologies, Vancouver, Canada) in a 15 mL tube and centrifuged at 800 × g for 20 min with brakes off.

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